Then, use anhydrous DMSO to dissolve FITC at 1 mg/ml. Do not use stored solutions of FITC in DMSO; prepare fresh on the day of the reaction. Step 2. Incubate Protein with FITC. Slowly and gently add 50 ul of FITC solution to each ml of protein solution in 5 ul intervals while stirring the mixture. Incubate the solutions in the dark at 4 °C for ... WebThey suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in DMSO) per 1 ml of protein solution. Final molar F/P ratio was 0.06. In another …
FITC, Isomer I Fluorescein Isothiocyanate, Isomer I 3326-32-7
WebDespite the growing number of choices in amine-reactive fluorophores, fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) are still widely used reactive fluorescent dyes for preparing fluorescent bioconjugates. ... (DMSO) and should never be used in that solvent. Figure 1.1.8. Reaction of a primary amine with a ... WebProtocols of Sigma and MPBio are the same. They suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in … bricktown elks lodge
Home - Fidelity Mechanical Services
WebMar 30, 2024 · 溶剂：溶于dmso、dmf，dcm，溶于水。 ... fitc-peg-nh2是一种线性异生物功能peg试剂，含有荧光素染料和胺基，与羟基甲酸、活化nhs酯、羰基（酮、醛）等反应。荧光素通常称为fitc，是生物学中常用的荧光示踪剂，用于染色细胞、组织、生物标记物或纳米 … WebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. WebFITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm. Optical Data for FITC. ... Soluble to 100 mM in DMSO. Purity: ≥95%. Storage: Store at -20°C. CAS No: … bricktown events mount union pa