Hepg2 electroporation
WebFirst, we tried to optimize the transfection of the hepatoma cell line HepG2 with PEX5 siRNA using different commercially available liposomal and non-liposomal transfection reagents … WebTip: HepG2 cells tend to grow in clusters. It is critical to ensure dissociation has occurred to yield a single-cell suspension when passaging and plating the cells. Having a single-cell …
Hepg2 electroporation
Did you know?
Web21 jun. 2024 · For some reasons, HepG2 are not so easy to transfect with plasmid DNA. We use them in-house routinely for testing our transfection reagents (Viromers). While siRNA … Web1 jan. 2009 · Electroporation is a physical method for cell transfection, where application of high voltage electric pulses is used to transiently permeabilize cell membranes allowing facilitated entry of...
WebAdd 1μg of plasmid DNA to the medium, and mix. For a 3:1 ViaFect™ Transfection Reagent:DNA ratio, add 3μl of ViaFect™ Transfection Reagent, and mix immediately. Incubate the ViaFect™ Transfection Reagent:DNA mixture for 5–20 minutes at room temperature. Optional: Add mixture to cells without an incubation period. WebThe exosomes harvested from these 293T cells were loaded with miR-26a via electroporation. Results: The engineered exosomes were shown to bind selectively to HepG2 cells via the scavenger receptor class B type 1–Apo-A1 complex and then internalized by receptor-mediated endocytosis. The release of miR-26a in exosome …
WebHepG2 is a human liver cancer cell line that was originally derived from a patient with hepatocellular carcinoma. Cell line is known for the sensitivity to a variety of chemotherapeutic agents, including cisplatin and … WebPassionate, motivated, and energetic scientist with over 13 years of pharmaceutical research and support experience seeking to utilize and expand extensive technical skills and knowledge to ...
WebThree commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two …
WebSpecies Human, HepG2, hepatoma Used DNA: supercoiled DNA used for transient transfections. Molecules Electroporated DMEM, 10% Fetal Calf Serum (FCS) … samsung health blood oxygen levelWebAdd 2 μg plasmid DNA. Pipet gently to mix. Add 2 μL X-tremeGENE™ HP DNA Transfection Reagent to the diluted DNA. ( 1:1 ratio of reagent to DNA). Pipet gently to mix. Incubate for 15 – 30 min at +15 °C to +25 °C. Add 10 μL transfection … samsung health blood oxygenWeb12 apr. 2024 · For CHO-K1 and HepG2 cells, the frequencies of EGFP-positive cells were high about 9.10–18.8%, with no significance among different reporter forms (Figure 3A,B). Even higher, up to 49.4%, EGFP-positive cells appeared in HepaRG cells with circular reporter plasmid, which was comparable with that in the EGFP-N2 positive control group … samsung health compatible scales 2020Web14 okt. 2024 · USP8 knockdown facilitates ferroptosis induced by erastin via ferritinophagy. (A) Knockdown of USP8 in MEF cells was verified by immunoblotting. (B) MEF cells were treated with indicated erastin for 24 h, cell viability was assayed using a CCK8 kit (n = 3).(C) MEF cells were treated with 0.5 μmol/L or 1 μmol/L erastin for 12 h, cell death was … samsung health compatible scales 2021Web31 okt. 2024 · Table 7 Gene ontology by component of the differently expressed proteins in the HepG2 the normal liver extracted with electroporation mapped with GOrilla 42. Full … samsung health data on pcWebFirst, we tried to optimize the transfection of the hepatoma cell line HepG2 with PEX5 siRNA using different commercially available liposomal and non-liposomal transfection reagents (Lipofectamine(®) 2000, FuGENE 6, HiPerFect(®), INTERFERin™, RiboJuice™) as well as microporation using the Neon™ Transfection system. samsung health compatible scales 2022WebElectroporation is a physical method for cell transfection, where application of high voltage electric pulses is used to transiently permeabilize cell membranes allowing … samsung health body temperature